- Title
- A comparative analysis of HIV-specific mucosal/systemic T cell immunity and avidity following rDNA/rFPV and poxvirus-poxvirus prime boost immunisations
- Creator
- Ranasinghe, Charani; Eyers, Fiona; Stambas, John; Boyle, David B.; Ramshaw, Ian A.; Ramsay, Alistair J.
- Relation
- Vaccine Vol. 29, Issue 16, p. 3008-3020
- Publisher Link
- http://dx.doi.org/10.1016/j.vaccine.2011.01.106
- Publisher
- Elsevier
- Resource Type
- journal article
- Date
- 2011
- Description
- In this study we have firstly compared a range of recombinant DNA poxvirus prime-boost immunisation strategies and shown that combined intramuscular (i.m.) 2× DNA-HIV/intranasal (i.n.) 2× FPV-HIV prime-boost immunisation can generate high-level of HIV-specific systemic (spleen) and mucosal (genito-rectal nodes, vaginal tissues and lung tissues) T cell responses and HIV-1 p24 Gag-specific serum IgG1, IgG2a and mucosal IgG, SIgA responses in vaginal secretions in BALB/c mice. Data indicate that following rDNA priming, two rFPV booster immunisations were necessary to generate good antibody and mucosal T cell immunity. This data also revealed that mucosal uptake of recombinant fowl pox (rFPV) was far superior to plasmid DNA. To further evaluate CD8+ T cell immunity, i.m. 2× DNA-HIV/i.n. 1× FPV-HIV immunisation strategy was directly compared with single shot poxvirus/poxvirus, i.n. FPV-HIV/i.m. VV-HIV immunisation. Results indicate that the latter strategy was able to generate strong sustained HIV-specific CD8+ T cells with higher avidity, broader cytokine/chemokine profiles and better protection following influenza-KdGag197–205 challenge compared to rDNA poxvirus prime-boost strategy. Our findings further substantiate the importance of vector selection/combination, order and route of delivery when designing effective vaccines for HIV-1.
- Subject
- poxvirus vectors; DNA vaccines; mucosal immunity; T cell avidity
- Identifier
- http://hdl.handle.net/1959.13/1066466
- Identifier
- uon:18118
- Identifier
- ISSN:0264-410X
- Language
- eng
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